In 2018 USFWS retained Environmental Solutions & Innovations, Inc. and Stone Environmental to complete bumble bee surveys and provide management recommendations to enhance pollinator habitat and populations at Missisquoi NWR. Survey methods followed the guidance issued by USFWS Ecological Services for rusty patched bumble bee, with an expansion to survey all bumble bee species and record time of collection. Survey objectives included an inventory of bumble bee species in habitats with high availability of floral resources, such as early successional grasslands/meadows/old fields or shrubby wetlands; on National Wildlife Refuges between 1 June and 15 September in areas with available floral resources. Sampling objectives for each survey site were: generate a bumble bee species list, measure bumble bee species richness and relative abundance by species, and explore habitat relationships with bumble bee richness and abundance. In 2018, bumble bee surveys were conducted at 12 sites, resulting in the detection of 992 individuals of 12 species.
此資源sampling event的資料已發佈為達爾文核心集檔案（DwC-A），其以一或多組資料表構成分享生物多樣性資料的標準格式。 核心資料表包含 29 筆紀錄。
亦存在 1 筆延伸集的資料表。延伸集中的紀錄補充核心集中紀錄的額外資訊。 每個延伸集資料表中資料筆數顯示如下。
下載最新版本的 Darwin Core Archive (DwC-A) 資源，或資源詮釋資料的 EML 或 RTF 文字檔。
Longenecker R, Hardy S, Richardson L, Sefchick J (2022): Missisquoi National Wildlife Refuge Bumblebee (Bombus) Surveys. v1.2. Vermont Center for Ecostudies. Dataset/Samplingevent. https://ipt.vtatlasoflife.org/resource?r=mnwrbombus&v=1.2
此資料的發布者及權利單位為 Vermont Center for Ecostudies。 This work is licensed under a Creative Commons Attribution (CC-BY) 4.0 License.
Missisquoi National Wildlife Refuge, Vermont, USA
|界定座標範圍||緯度南界 經度西界 [44.916, -73.225], 緯度北界 經度東界 [45.013, -73.103]|
Bumblebee counts and Honey Bee presence/absence
|Species||Apis mellifera (Western Honey Bee)|
|起始日期 / 結束日期||2018-05-23 / 2018-09-18|
Survey methods follow USFWS RPBB Survey Protocol guidance (USFWS 2018a) and were subsequently expanded to survey all bumblebee species and record time of collection (Longenecker et al. 2018).
|研究範圍||Surveys were conducted at 12 sites within MNWR between late May and September of 2018.|
- Development of Initial Survey Instructions Initial Survey Instructions (ISI) were developed through coordination among the USFWS (Dr. Rebecca Longenecker, Laura Eaton, and Maritza Mallek), ESI (Dr. Robert Jean), and SE (Dr. Leif Richardson) via conference calls, email, and document sharing programs. The ISI follows the survey protocol format developed by the USFWS Inventory and Monitoring Program. Survey methods follow USFWS RPBB Survey Protocol guidance (USFWS 2018a) and were subsequently expanded to survey all bumble bee species and record time of collection (Longenecker et al. 2018). The ISI ensure survey protocol and procedural uniformity across Region 5 NWRs and serve as a model for future surveys and for other NWR regions. The ISI, and all data and reports from this project, are available at https://ecos.fws.gov/ServCat/Reference/Profile/109238. Guidance for RPBB Survey Protocol is regularly updated by USFWS at https://ecos.fws.gov/ServCat/Reference/Profile/104984.
- Habitat and Site Selection Old field, meadow, open grassy areas, and ecotones along forests comprise preferred bumble bee habitats as the areas provide abundant foraging (flowering), and possibly nesting and overwintering habitat. Marshlands and wetlands are potentially used extensively at certain times of year when large amounts of nectar and pollen are available. Based upon species’ preference, SE, USFWS, and MNWR staff selected survey sites within approximately 223 acres (90 ha) of grasslands, marshlands, and wetlands. For clarity, mapping for the eastern and western segments of the MNWR are provided on Figures 2 and 3, respectively in Appendix A and the specific map depicting each site is provided in Table 1. Overall, 12 sites were selected (Table 1; Appendix A, Figures 2 and 3) with 9 sampled during the first sampling period, ten in the second sampling period, and ten sites in the third sampling period. Eight sites (Louie’s Landing, Rte 78S, RT Wetland, S. Young Marsh, Tabor E-01, Tabor E-03, Tabor W-01 and Tabor W-02), were sampled across all three time periods. One site, Tabor E-02, was sampled during two time periods and three sites (Cranberry Pool Dike, Rte 78 N, and Other) were sampled during a single sampling period. Sample sites encompassed a maximum of four acres (1.6 ha) in area (Longenecker et al. 2018).
- Habitat Assessment A desktop analysis was completed to determine the approximate amount and quality of available habitat potentially suitable for use by pollinators, particularly bumble bees, within the 6,729-acre (2,723-ha) project area. Data acquired for this work include (but are not limited to): historical mapping; historical imagery of the project area using Google Earth mapping; and other readily available commercial, institutional, and governmental sources. Other information includes soil and geologic information and distributional and conservation data for target species and associated habitats and plant communities. On-site habitat assessments followed guidance provided by federal and state agencies and previous bumble bee collection experience. Assessments used a combination of available technology including GIS systems (ESRI ArcMap), handheld GPS units, tablet computers, and programs written by USFWS staff to ensure a high quality, easily interpretable, and universal standard of mapping for field studies and reporting for the target species. This approach avoids some common issues including inconsistent data entry and multiple and incongruous GIS files. As data are collected in the field, QA/QC analysis is completed in “real time”. For this study, initial mapping and associated databases were provided by USFWS. Results of desk-top analysis and on-site habitat assessments were used to guide field surveys.
- Field Surveys Bumble bee presence/absence surveys, with an emphasis on RPBB, were completed at each survey site across three time (survey) periods for three days each in May, June, and September following approved techniques (USFWS 2018a) and the ISI (Longenecker et al. 2018). Survey techniques included: using binoculars to monitor potential resource patches, using aerial nets to capture specimens, temporarily placing specimens in clear containers, photographing, and releasing specimens. Attempts were made to catch all bumble bees observed in survey plots. A standard 15-inch (0.38-m) aerial insect net with a two- to four-foot (0.61- to 1.22-m) handle was used. Sample areas were delineated, environmental and ecological data were recorded, and all bumble bee species observed were documented, including time of capture and flower species where a bee is collected (if foraging). Sampling for bumble bees occurred outside the optimal window for surveys (15 June – 15 August) based on a special request from USFWS to accommodate refuge and surveyor schedules when bumble bees were still active. At MNWR, samples were collected by two or three surveyors for 60 minutes at each sample site. Further details are provided in the ISI (Longenecker et al. 2018). Efforts were made to sample bees under the following environmental conditions: • Temperatures > 60o Fahrenheit (15.6o Celsius) • < 80 percent cloud cover • Light winds • No rain